Zebrafish smyhc1 Knockdown Using Morpholino

The Morpholino antisense oligo against zebrafish smyhc1 was synthesized by Gene Tools (Corvalis, OR). The smyhc1 translation blocker (ATG-MO, Table 1) was designed to target the sequence flanking the ATG start site (32 (link)). The standard control-MO was purchased from Gene Tools. The Morpholino antisense oligos were dissolved with 1× Danieau buffer (39 (link)) to a final concentration of 0.5mM. Zebrafish embryos at the 1 or 2 cell stage were injected with approximately 2 nl (10 ng) of the ATG-MO.
smyhc1 ATG-MO: 5’-TCTAAAGTTTTACCCACTGCGGCAA-3’

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Li S., Wen H, & Du S. (2019). Defective sarcomere organization and reduced larval locomotion and fish survival in slow muscle heavy chain 1 (smyhc1) mutants. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(1), 1378-1397.

Publication 2019

Morpholino antisense oligo standard control-MO

Buffer Cell Embryos Gene Morpholino oligos Oligo Zebrafish

Corresponding Organization :

Other organizations : University of Maryland, Baltimore, Ocean University of China

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Variable analysis

independent variables

Morpholino antisense oligo against zebrafish smyhc1 (ATG-MO)

dependent variables

Outcome of injecting zebrafish embryos with ATG-MO

control variables

Zebrafish embryos at the 1 or 2 cell stage injected with the standard control-MO

controls

Positive control: None mentioned
Negative control: Zebrafish embryos injected with the standard control-MO

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