Glioblastoma Cell Lines Treated with Compounds

Four human glioblastoma cell lines, U87 (ATCC HTB-14), LN18 (ATCC CRL-2610), U251 and SF767 (obtained from M. Celeste Simon’s laboratory, University of Pennsylvania, Philadelphia, PA, USA), were used and routinely maintained in Dulbecco’s Modified Eagle Medium (DMEM, Lonza) supplemented with 10% fetal calf serum (Lonza) at 37C° in 5% CO₂-humidified incubators and were subcultured once or twice a week as described previously [20 (link)]. Cells were treated or not with the following compounds, 1-10mM metformin (Sigma-Aldrich), 5–100μM temozolomide (Schering-Plough Europe), 10μM bafilomycin (Sigma-Aldrich) and irradiated or not at 5Gy using the Gammacell 40 Exactor irradiator (Nordion).