Establishing Xenograft and Intracranial Glioblastoma Models

Animal experiments were performed in accordance with a study protocol approved by the Institutional Animal Care and Use Committee of the University of Tennessee Health Science Center. Xenografts were established in five-week-old male NOD.Cg-Prkdc^scidIl2rg^tm1Wjl/SzJmice (Jackson Laboratory) by injection of MT330 and SJG2 cells (1x10⁶) directly into the flanks [37 (link)]. Tumors were measured weekly with a handheld caliper. In addition, luciferase-expressing GBM cells (10⁶) were injected stereotactically into the superficial brain parenchyma of NSG mice through a burr hole in the skull as previously described [49 (link)]. NSG mice were injected with D-luciferin and subjected to live animal imaging weekly to quantify bioluminescence [38 (link), 49 (link)].

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Yang C.H., Wang Y., Sims M., Cai C., He P., Yue J., Cheng J., Boop F.A., Pfeffer S.R, & Pfeffer L.M. (2016). MiRNA203 suppresses the expression of protumorigenic STAT1 in glioblastoma to inhibit tumorigenesis. Oncotarget, 7(51), 84017-84029.

Publication 2016

NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJmice

Animal Brain Burr hole Cells Institutional animal care and use committee Luciferase Luciferin Male Mice Skull Tumors Xenografts

Corresponding Organization : University of Tennessee Health Science Center

Other organizations : China Medical University

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Protocol cited in 2 other protocols

Variable analysis

independent variables

Injection of MT330 and SJG2 cells (1x10^6) directly into the flanks of five-week-old male NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ mice (Jackson Laboratory)
Injection of luciferase-expressing GBM cells (10^6) stereotactically into the superficial brain parenchyma of NSG mice through a burr hole in the skull

dependent variables

Tumor measurements using a handheld caliper
Bioluminescence quantification in NSG mice injected with D-luciferin and subjected to live animal imaging weekly

control variables

Five-week-old male NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ mice (Jackson Laboratory)
NSG mice

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