Quantitative PCR Statistical Analysis

Statistical analysis of the qPCR results was performed in analogy to the described earlier method^{53 (link)}. PCR efficiency of each reaction was ranged from 92% to 99%. The results from the biological replicates for particular samples were gathered to determine the mean normalized expression and its standard deviation (Bio-Rad CFX Manager 3.0). The normalized relative expression of ZsGreen1 from biological replicates for siRNAs and control siRNA was compared at a significance level of 0.05 or 0.01 using Bio-rad CFX Manager 3.0. Statistically significant differences in mean expression between tested siRNAs and control siRNA (P < 0.05 or P < 0.01) were observed but not in all samples.

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Lisowiec-Wąchnicka J., Bartyś N, & Pasternak A. (2019). A systematic study on the influence of thermodynamic asymmetry of 5′-ends of siRNA duplexes in relation to their silencing potency. Scientific Reports, 9, 2477.

Publication 2019

CFX Manager 3.0

Biological Sirna

Corresponding Organization :

Other organizations : Institute of Bioorganic Chemistry, Polish Academy of Sciences

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Variable analysis

independent variables

SiRNAs

dependent variables

Normalized relative expression of ZsGreen1

control variables

Control siRNA

controls

Positive control: Not explicitly mentioned.
Negative control: Control siRNA

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