Immunohistochemical stainings were performed as described in19 (link). Primary antibodies used are anti-Troponin T cardiac isoform (Thermo Scientific); anti-CD45, α-SMA, anti-Cytokeratin 10 and anti-Ki 67 (all from Abcam). For detection of binding of the primary antibody, the Ultra-Sensitive ABC Peroxidase rabbit/mouse IgG staining kit (Thermo Scientific, Waltham, MA, USA) and the ImmPACT VIP-Peroxidase substrate kit (Vector, Burlingame, CA, USA) were used according to the respective manufacturer’s protocols. Nuclei were counterstained with hematoxylin and slides were dehydrated, cleared in xylene, and mounted with Eukitt (Sigma Aldrich, USA).
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