Venom Procoagulant Activity Assay

Data are presented as mean ± SD. Graphics were generated with a commercially available program (Origin2020b, OriginLab Corporation, Northampton, MA, USA). Experimental conditions were composed of n = 6 replicates per condition as this provides a statistical power > 0.8 with p < 0.05 utilizing these techniques [1 (link),2 (link),3 (link),4 (link)]. A statistical program was used for one-way analyses of variance (ANOVA) comparisons between conditions, followed by Holm–Sidak post hoc analysis. Additional analysis with two-way ANOVA was performed to detect significant interactions between CORM-2 and RuCl₃, CORM-3 and RuCl₃, and CORM-2 and carboplatin regarding changes in venom procoagulant activity. All analyses were performed with commercial software (SigmaPlot 14, Systat Software, Inc., San Jose, CA, USA). p < 0.05 was considered significant.