Microscopic Imaging of Intestinal and Salivary Infections

The smears from infected intestine and salivary glands were fixed for 30 min with ethanol and stained with either Giemsa or 4’,6-diamidino-2-phenylindole (DAPI) as described before [35 (link),36 (link)]. Digital images were acquired in DM 2500 microscope (Leica Microsystems GmbH, Wetzlar, Germany) equipped with UCMOS14000KPA 14-Mpx camera (Toup Tek, Hangzhou, China) at ×1,000 magnification. All measurements of cells (n = 25) and statistical analysis were performed in UTHSCSA Image Tool for Windows v. 3.0. For transmission and scanning electron microscopy the samples were fixed and processed as described previously [37 (link)].

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Frolov A.O., Malysheva M.N., Ganyukova A.I., Spodareva V.V., Yurchenko V, & Kostygov A.Y. (2019). Development of Phytomonas lipae sp. n. (Kinetoplastea: Trypanosomatidae) in the true bug Coreus marginatus (Heteroptera: Coreidae) and insights into the evolution of life cycles in the genus Phytomonas. PLoS ONE, 14(4), e0214484.

Publication 2019

DM 2500 microscope

Cells Ethanol Intestine Microscope Salivary glands Scanning electron microscopy Transmission

Corresponding Organization : Sechenov University

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Variable analysis

independent variables

Fixation time (30 min)
Staining method (Giemsa or DAPI)

dependent variables

Microscopic observations of cells (cell measurements, n = 25)
Electron microscopy (transmission and scanning)

control variables

Ethanol fixation
Microscope (DM 2500 with UCMOS14000KPA 14-Mpx camera)
Magnification (×1,000)
Image analysis software (UTHSCSA Image Tool for Windows v. 3.0)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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