Pharmacological Modulation of Cellular Transport

Brefeldin A (BFA, REF: 3660755, 10-40nM [24 (link), 32 (link)]), thapsigargin (TG, REF: 3557977, 0.1-100nM [33 (link), 34 (link)]), wortmannin (WORT, REF: W3144, 1µM [35 (link), 36 (link)]) and cytochalasin D (CytD, REF: C2618, 100nM [37 (link), 38 (link)]) were purchased from Sigma-Aldrich (Burlington, Massachusetts, United States). Stock solutions of the compounds were prepared in dimethyl sulfoxide (DMSO; Carl Roth, Karlsruhe, Germany) to enable a 1:1000 dilution for each treatment. Solvent controls were matched to represent the same DMSO content as the treatments. PBS-A was prepared by dissolving 0.4g KH₂PO₄, 0.4g KCl, 16g NaCl and 5.5g Na₂HPO₄. 2H₂O in 1l autoclaved water and adjusting the pH to 7.2.

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Gruber L., Jobst M., Kiss E., Karasová M., Englinger B., Berger W, & Del Favero G. (2023). Intracellular remodeling associated with endoplasmic reticulum stress modifies biomechanical compliance of bladder cells. Cell Communication and Signaling : CCS, 21, 307.

Publication 2023

Brefeldin A thapsigargin wortmannin cytochalasin D (CytD DMSO

Brefeldin a Cytochalasin d Dilution Dmso Nacl Solvent Thapsigargin Wortmannin

Corresponding Organization :

Other organizations : University of Vienna, Comprehensive Cancer Center Vienna, Medical University of Vienna

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Variable analysis

independent variables

Brefeldin A (BFA)
Thapsigargin (TG)
Wortmannin (WORT)
Cytochalasin D (CytD)

dependent variables

Not explicitly mentioned

control variables

Solvent (DMSO) controls
PBS-A (phosphate-buffered saline)

controls

Solvent (DMSO) controls matched to represent the same DMSO content as the treatments
PBS-A (phosphate-buffered saline) prepared by dissolving specific reagents and adjusting the pH to 7.2

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