Quantifying Urinary Adherence Markers

To measure urinary concentrations of the adherence marker compounds, urine samples were thawed at room temperature (25°C) for 2 h and diluted 10 (trans-resveratrol) or 50 (saccharin) times in methanol:water (1:1). Urine samples containing saccharin had 100 ng/mL of saccharin-d4 (CDN Isotopes) added, and were vortexed for 5 s. Samples were transferred to an autosampler vial for analysis by Agilent 1290 Infinity Ultra-HPLC (UHPLC) interfaced to a 6460 triple-quadrupole mass spectrometer (MS/MS) with electrospray ionization (ESI) via Jet Stream technology (Agilent Technologies) (15 (link)). Resveratrol metabolites were expressed as resveratrol equivalents. Recoveries for this method have been shown to be >80% at the range of concentrations used in this study.

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Zyba S.J., Weinborn V., Arnold C.D., Lehmkuhler A.L., Morel F.B., Zeilani M., Mitchell A.E, & Haskell M.J. (2021). Evaluation of Saccharin and Resveratrol as Extrinsic Markers of Small-Quantity Lipid-Based Nutrient Supplement Consumption in Healthy Women. Current Developments in Nutrition, 5(7), nzab089.

Publication 2021

saccharin-d4 Jet Stream technology

Hplc Isotopes Methanol Resveratrol Saccharin Trans resveratrol Urine

Corresponding Organization : University of California, Davis

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Variable analysis

independent variables

Dilution factor (10x for trans-resveratrol, 50x for saccharin)

dependent variables

Urinary concentrations of the adherence marker compounds (trans-resveratrol and saccharin)

control variables

Urine sample thawing temperature (25°C)
Urine sample thawing duration (2 h)
Methanol:water ratio (1:1) for dilution
Saccharin-d4 concentration (100 ng/mL) for samples containing saccharin
Vortex duration (5 s) for samples containing saccharin

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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