Neuronal Activation and cFos Induction

To stimulate neuronal activation-induced cFos expression in the hippocampus, mice were subjected to a complex running wheel (CRW) set-up for 4 h [43 (link)]. Mice were single housed in type III cages (42 × 26 × 18 cm, Tecniplast), equipped with CRW (TSE-Systems) characterized by randomized omitted bars [26 (link), 27 (link)]. Mice were habituated to the experimental room and CRW for 2 h prior to dark phase. After dark phase onset, voluntary running was automatically tracked for 4 h via Phenomaster software (TSE-Systems) and the total running distance per mouse calculated. Mice were perfused with Ringer and 4% formaldehyde/PBS, 30 min after removal from the CRW set-up.

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Wilke J.B., Hindermann M., Moussavi A., Butt U.J., Dadarwal R., Berghoff S.A., Sarcheshmeh A.K., Ronnenberg A., Zihsler S., Arinrad S., Hardeland R., Seidel J., Lühder F., Nave K.A., Boretius S, & Ehrenreich H. (2021). Inducing sterile pyramidal neuronal death in mice to model distinct aspects of gray matter encephalitis. Acta Neuropathologica Communications, 9, 121.

Publication 2021

Phenomaster software

Formaldehyde Hippocampus Mice Neuronal

Corresponding Organization :

Other organizations : Max Planck Institute of Experimental Medicine, German Primate Center, University of Göttingen, Universitätsmedizin Göttingen

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Variable analysis

independent variables

Exposure to complex running wheel (CRW) set-up for 4 hours

dependent variables

Neuronal activation-induced cFos expression in the hippocampus

control variables

Mice were single housed in type III cages (42 × 26 × 18 cm, Tecniplast), equipped with CRW (TSE-Systems) characterized by randomized omitted bars
Mice were habituated to the experimental room and CRW for 2 h prior to dark phase
Voluntary running was automatically tracked for 4 h via Phenomaster software (TSE-Systems) and the total running distance per mouse calculated
Mice were perfused with Ringer and 4% formaldehyde/PBS, 30 min after removal from the CRW set-up

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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