The Ubi and the mutant plasmids were described in our previous study [53 (link)]. Wild type (WT) and mutant USP8 or β-catenin plasmids were obtained from Hanbio Biotechnology Co., Ltd. (Shanghai, China). The Small interfering RNAs (siRNAs) targeting USP8 (siRNA-1: 5′- GGCAAGCCAUUUAAGAUUA-3′; 5′- CCACUAGCAUCCACAAGUA-3′) were obtained from Genepharma (Shanghai, China).
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