Anti-FLAG antibody was purchased from eBioscience (Cat#14-6681-82), anti-panCK antibody
from Biogenex (Cat#AM273-5 M), anti-VHL antibody from Abcam (Cat#ab140989), and
anti-CK8/18 from Novus (Cat#NBP2-44929). Murine ccRCC cell line RENCA and human ccRCC cell
line ACHN, prostate cancer cell lines CWR22v1 and C4-2 were purchased from the American
Type Culture Collection (ATCC) and maintained in RPMI-1640, supplemented with 10% fetal
bovine serum and penicillin/Streptomycin at a working concentration of 100 U/mL. Murine
prostate cancer cells Myc-CaP were purchased from ATCC and maintained in DMEM,
supplemented with 10% fetal bovine serum and penicillin/Streptomycin at a working
concentration of 100 U/mL. Human bladder cancer cell lines T24 and HT-1376 were kind gifts
from Dr. Arnold I. Chin and Dr. Hanwei Zhang at UCLA and maintained in the same condition
as RENCA cells.
Lentiviral plasmid encoding mStrawberry and EGFP, together with flag tag or HA, and
plasmid encoding firefly luciferase were constructed based on pSicoR (Addgene, #11579),
and lentivirus was packaged as mentioned previously in the report26 (link).
from Biogenex (Cat#AM273-5 M), anti-VHL antibody from Abcam (Cat#ab140989), and
anti-CK8/18 from Novus (Cat#NBP2-44929). Murine ccRCC cell line RENCA and human ccRCC cell
line ACHN, prostate cancer cell lines CWR22v1 and C4-2 were purchased from the American
Type Culture Collection (ATCC) and maintained in RPMI-1640, supplemented with 10% fetal
bovine serum and penicillin/Streptomycin at a working concentration of 100 U/mL. Murine
prostate cancer cells Myc-CaP were purchased from ATCC and maintained in DMEM,
supplemented with 10% fetal bovine serum and penicillin/Streptomycin at a working
concentration of 100 U/mL. Human bladder cancer cell lines T24 and HT-1376 were kind gifts
from Dr. Arnold I. Chin and Dr. Hanwei Zhang at UCLA and maintained in the same condition
as RENCA cells.
Lentiviral plasmid encoding mStrawberry and EGFP, together with flag tag or HA, and
plasmid encoding firefly luciferase were constructed based on pSicoR (Addgene, #11579),
and lentivirus was packaged as mentioned previously in the report26 (link).
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