Pharmacological Modulation of Cortical Perfusion

Drug solutions or vehicle of equal volume (1.5% DMSO in 10 ml aCSF) were superfused on the cortical surface free of dura in the rostral cranial window after taking DC potential and CBF baseline for 5 min under aCSF. Drug concentrations were carefully selected based on dose response curves, selectivity and efficacy reported previously14 (link)16 (link)18 (link)34 (link). The following drugs were applied topically: the selective COX-2 inhibitor NS-398 (100 μM; Cayman)16 (link), the selective COX-1 inhibitor SC-560 (25 μM; Cayman)22 (link), or the selective PGE₂ receptor (EP4) antagonist L161,982 (1 μM; Sigma)14 (link)34 (link). The pharmacological treatment was initiated 40 min prior ischemia induction or the elicitation of the first SD event (i.e. sham-operated group)16 (link) and incubation persisted till the end of the experimental protocol.

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Varga D.P., Puskás T., Menyhárt Á., Hertelendy P., Zölei-Szénási D., Tóth R., Ivánkovits-Kiss O., Bari F, & Farkas E. (2016). Contribution of prostanoid signaling to the evolution of spreading depolarization and the associated cerebral blood flow response. Scientific Reports, 6, 31402.

Publication 2016

NS-398 SC-560 L161,982

Cayman Cortical Cox 1 Cox 2 inhibitor Cranial Dmso Drug solutions Drugs Dura Ischemia Ns 398 Pge2 Pharmacological treatment Sc 560

Corresponding Organization :

Other organizations : University of Szeged

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Variable analysis

independent variables

Drug solutions or vehicle of equal volume (1.5% DMSO in 10 ml aCSF) superfused on the cortical surface
The selective COX-2 inhibitor NS-398 (100 μM)
The selective COX-1 inhibitor SC-560 (25 μM)
The selective PGE2 receptor (EP4) antagonist L161,982 (1 μM)

dependent variables

DC potential
CBF (Cerebral Blood Flow)

control variables

Baseline measurements taken for 5 min under aCSF prior to drug application
Pharmacological treatment initiated 40 min prior to ischemia induction or the elicitation of the first SD event in the sham-operated group

controls

Positive control: Vehicle of equal volume (1.5% DMSO in 10 ml aCSF) superfused on the cortical surface
Negative control: Not explicitly mentioned

Annotations

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