Murine Hepatic Ischemia-Reperfusion Injury

Partial (70%) warm hepatic I/R in mice was performed as previously described (27 (link)). After 60 min of liver ischemia by vascular occlusion, the clip was carefully removed, and mice were reperfused and euthanized immediately at 0, 6, 12, or 24 h post-reperfusion. Blood and liver tissue samples were collected from anesthetized mice and were then preserved in liquid nitrogen for further phenotypic analyses. Sham controls were subjected to the same operation procedure but without clamping. For antibody treatment, approximately 1 h before hepatic I/R, mice were administered with isotype antibody (A110-1, BD Biosciences, San Jose city, CA, USA) (800 ug) or P-selectin (RB40.34, Thermo Scientific, Waltham, MA, USA) (200 ug), E-selectin (BE0294, BioXcell, Lebanon, NH, USA) (200 ug), and αvβ3 integrin antibody (RMV-7/2C9.G2, Biolegend, San Diego, CA, USA) (200/200 ug) via intraperitoneal injection(s).