Quantification of Angiogenic Factors in mdx Mice

TaqMan Gene Expression Assays (Thermofisher, Waltham, MA, USA) were used to assay Tek (Tie2) (Mm004432343_m1), Angpt1 (Mm00456503_m1), Angpt2 (Mm00545822_m1) [17 (link)], ActB (Mm02619580_g1), AP3D1 (Mm00475961_m1), and GusB (Mm01197698_m1). qPCR products were generated using the QuantStudio^TM 5 system with TaqMan Fast Advanced Master Mix (Applied Biosystems, Waltham, MA, USA). Quantification was performed using Design & Analysis Software v2.4.3 (Thermofisher, Waltham, MA, USA). Expression of Tek, Angpt1, and Angpt2 was normalized to the geometric mean of control genes (ActB, AP3D1, and GusB) validated for use in mdx mice [18 (link)].

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Lin Y., McClennan A, & Hoffman L. (2023). Characterization of the Ang/Tie2 Signaling Pathway in the Diaphragm Muscle of DMD Mice. Biomedicines, 11(8), 2265.

Publication 2023

TaqMan Gene Expression Assays QuantStudioTM 5 system TaqMan Fast Advanced Master Mix

Angpt1 Angpt2 Assay Gene expression Genes control Mdx mice

Corresponding Organization : Lawson Health Research Institute

Top 5 similar protocols

Variable analysis

independent variables

Tek (Tie2)
Angpt1
Angpt2

dependent variables

Expression of Tek
Expression of Angpt1
Expression of Angpt2

control variables

AP3D1

controls

The expression of Tek, Angpt1, and Angpt2 was normalized to the geometric mean of the control genes (ActB, AP3D1, and GusB), which were validated for use in mdx mice.

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