After appendectomy, a gauge was inserted in the caecal side of the lumen of the appendix and 3 mL of saline 0.9% was administered and collected, and re-administered and re-collected 3 additional times.
Previous training was carried out for appendicular lavage so that the process was standardized and data collection uniformised. A tutorial was presented to all the surgeons that performed an appendectomy. The appendicular fluid samples were collected with Sarstedt Monovette tubes and centrifuged. A total of 1 mL of the supernatant was extracted and stored at −20 °C [5 (link)].
Previous training was carried out for appendicular lavage so that the process was standardized and data collection uniformised. A tutorial was presented to all the surgeons that performed an appendectomy. The appendicular fluid samples were collected with Sarstedt Monovette tubes and centrifuged. A total of 1 mL of the supernatant was extracted and stored at −20 °C [5 (link)].
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