Phrenic Nerve Conduction Study in Rats

Rats were anesthetized with isoflurane (Piramal Healthcare) at a concentration of 3.0–3.5% diluted in oxygen. Animals were placed supine, and the region just below the rib cage was shaved and cleaned with 70% ethanol. Phrenic nerve conduction studies were performed with stimulation of the phrenic nerve via needle electrodes transcutaneously inserted into the neck of the rat in proximity to the passage of the phrenic nerve (Lepore et al., 2008 (link), 2010 (link)). A reference electrode was placed on the shaved surface of the right costal region. The phrenic nerve was stimulated with a single burst at 6 mV (amplitude) for a 0.5-ms duration. Each animal was stimulated between 10 and 20 times to ensure reproducibility, and recordings were averaged for analysis. ADI Powerlab8/30 stimulator and BioAMP amplifier (ADInstruments) were used for both stimulation and recording, and Scope 3.5.6 software (ADInstruments; RRID: SCR_001620) was used for subsequent data analysis. Following recordings, animals were immediately euthanized with a dose of Euthasol, and tissue was collected (as described below).

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Urban M.W., Ghosh B., Block C.G., Strojny L.R., Charsar B.A., Goulão M., Komaravolu S.S., Smith G.M., Wright M.C., Li S, & Lepore A.C. (2019). Long-Distance Axon Regeneration Promotes Recovery of Diaphragmatic Respiratory Function after Spinal Cord Injury. eNeuro, 6(5), ENEURO.0096-19.2019.

Publication 2019

ADI Powerlab8/30 stimulator BioAMP amplifier Scope 3.5.6 software

Animals Costal Ethanol Isoflurane Neck Needle Nerve conduction studies Oxygen Phrenic nerve Rib cage Tissue

Corresponding Organization :

Other organizations : Thomas Jefferson University, Temple University, Arcadia, Arcadia University

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Variable analysis

independent variables

Concentration of isoflurane (3.0–3.5%) used to anesthetize the rats

dependent variables

Phrenic nerve conduction
Recordings of phrenic nerve stimulation

control variables

Placement of rats in supine position
Shaving and cleaning the region below the rib cage with 70% ethanol
Stimulation of the phrenic nerve via needle electrodes transcutaneously inserted into the neck of the rat
Placement of a reference electrode on the shaved surface of the right costal region
Stimulation of the phrenic nerve with a single burst at 6 mV (amplitude) for a 0.5-ms duration
Number of stimulations (10-20 times) to ensure reproducibility
Use of ADI Powerlab8/30 stimulator and BioAMP amplifier for stimulation and recording
Use of Scope 3.5.6 software for data analysis

positive controls

No positive controls were explicitly mentioned in the input protocol.

negative controls

No negative controls were explicitly mentioned in the input protocol.

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