ERG measurements were done as described previously (Kim et al., 2017 (link)). In brief, mice were either dark- or light-adapted for 12 h and anesthetized with 2,2,2-tribromoethanol (Sigma, USA) in prior to dilating the pupils of the mice by 0.5% tropicamide. The mice were placed with a gold-plated objective lens on their corneas and silver-embedded needle electrodes at their foreheads and tails. The ERG recordings were performed using Micron IV retinal imaging microscope (Phoenix Research Labs, USA) and analyzed by Labscribe ERG software according to the manufacturer’s instruction.
Mouse visual acuity was measured with the OptoMotry system (Cerebral Mechanics, USA) as previously described (Prusky et al., 2004 (link)). Mice adapted to ambient light for 30 min were placed on the stimulus platform surrounded by four computer monitors displaying black and white vertical stripe patterns. An event that mice track the stripe movements with reflexive head-turn was counted as a successful visual detection. The detection thresholds were then obtained from the OptoMotry software.