PEP-1-PEBP1 Protects Cells from H2O2-Induced Toxicity
Effects of PEP-1-PEBP1 or control-PEBP1 protein on cell viability was assessed based on a WST-1 assay kit (Daeillab Service, Seoul, South Korea) as described previously [15 (link)]. Briefly, PEP-1-PEBP1 or control-PEBP1 protein (0.5–3 μM) was pretreated in NSC34 cells for 1 h and thereafter cells were exposed to 1 mM H2O2 for 5 h. Cell viability was measured at 450 nm using an ELISA microplate reader (Labsystems Multiskan MCC/340, Helsinki, Finland), and cell viability was expressed as a percentage value versus that in the untreated control cells.
Kim W., Cho S.B., Jung H.Y., Yoo D.Y., Oh J.K., Choi G.M., Cho T.G., Kim D.W., Hwang I.K., Choi S.Y, & Moon S.M. (2019). Phosphatidylethanolamine-Binding Protein 1 Ameliorates Ischemia-Induced Inflammation and Neuronal Damage in the Rabbit Spinal Cord. Cells, 8(11), 1370.
Corresponding Organization : Hallym University Dongtan Sacred Heart Hospital
Other organizations :
Seoul National University, Soonchunhyang University, Hallym University Sacred Heart Hospital, Chuncheon Sacred Heart Hospital, Hallym University Kangnam Sacred Heart Hospital, Gangneung–Wonju National University
Cell viability measured at 450 nm using an ELISA microplate reader
control variables
Pretreatment duration of 1 h
Exposure to 1 mM H2O2 for 5 h
controls
Untreated control cells
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