SEM was performed, as previously described (4 (link)). Briefly, PLVs were fixed in 4% paraformaldehyde/2.5% glutaraldehyde/0.1M cacodylate overnight, post-fixed in buffered 1% osmium tetroxide, dehydrated, critically point dried, mounted onto aluminum stubs and sputtercoated with gold prior to imaging using a Zeiss Auriga FE SEM. Three SEM micrographs per sample group were randomly chosen for descriptive analysis.
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