Primary human monocytes were isolated and pooled from buffy coats of four healthy blood donors (New York Blood Service, Long Island, NY) by positive selection using MACS CD14 microbeads (Miltenyi Biotec) as described in our previous study (33 (link)). The monocytes were differentiated into MDMs in the presence of 5 ng/ml human granulocyte-macrophage colony-stimulating factor (Miltenyi Biotec) for 7 days prior to respective experiments.
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