Urea (99%), citric acid (99.5%), N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) (99%), N-hydroxysuccinimide (NHS) (98%), ethanol (99.9%), phosphate buffered saline (PBS) pH 7.4, anhydrous N,N-dimethylformamide (DMF), rhodamine (99.5%), copper (II) sulfate (99%), ascorbic acid (99.5%), azide-PEG300-biotin, irinotecan hydrochloride (IT), 4-Pentynoic acid, and Sephadex G10, G15, and G25, dialysis tubing MWCO 2 kDa were purchased from Sigma Aldrich and used as received. NH2-PEG2000-CC was obtained as previously described [29 (link)].
MCF-7 and MDA-MB-231 cell lines were purchased from Sigma Aldrich and cultured in supplemented Dulbecco’s Minimum Essential Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, EuroClone, Milan, Italy), 1% of penicillin/streptomycin (10,000 U mL−1 and 10 mg mL−1 respectively, EuroClone), and 1% ofl -glutamine (EuroClone), at 37 °C in 5% CO2 humidified atmosphere. Cell Titer 96 Aqueous One Solution Cell Proliferation assay (MTS solution) was purchased from Promega (Madison, WI, USA).
MCF-7 and MDA-MB-231 cell lines were purchased from Sigma Aldrich and cultured in supplemented Dulbecco’s Minimum Essential Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, EuroClone, Milan, Italy), 1% of penicillin/streptomycin (10,000 U mL−1 and 10 mg mL−1 respectively, EuroClone), and 1% of
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