Randomized Murine Microbiome Analysis

Collection of human samples was randomized and blinded. No other data collection and analysis was performed blind to the conditions of the experiments. No statistical methods were used to pre-determine sample sizes, but our sample sizes are similar to those reported in previous publications^{20 (link),72 (link),73 (link)}. Mice were randomly assigned to respective treatment groups. No animals or data points were excluded from the analyses. Data collection, statistical analysis and visualization utilized Microsoft Excel (22.03) GraphPad Prism (9.2.0), CorelDRAW 2019 (21.0.0.593) and in-house R scripts (3.6.2). The following packages were used with R (3.6.2): ggplot2 (2.3.5), qiime2R (0.99.6), dplyr (1.0.8), tidyverse (1.3.1) and RStudio (1.2.5033). Shapiro–Wilk normality test was applied to all datasets n > 6; for all other sets, we assumed non-normal distribution, but this was not formally tested. Testing for statistical significance always employed two-tailed tests if not stated otherwise.