Immunofluorescence Staining of Neural Markers
Corresponding Organization : Capital Medical University
Other organizations : First Affiliated Hospital of Wannan Medical College, Wannan Medical College, Huawei Technologies (China)
Variable analysis
- Primary antibodies against Iba-1, myeloperoxidase (MPO), 8-hydroxydeoxyguanosine (8-OHdG), Nrf2, caspase-1 p20, and NeuN
- Fluorescently stained cells recorded using ImageJ program
- Triton X-100 concentration (0.1%)
- BSA blocking concentration (5%)
- Incubation time for primary antibodies (overnight at 4°C)
- Microscope system (ZEISS HB050 inverted microscope)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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