Inducible Expression of OCT1-3 in HEK293 Cells

Jump In T-Rex human embryonic kidney 293 cells (HEK293-JumpIn) with doxycycline-inducible expression of human OCT1, OCT2 or OCT3 (HEK293-JI-OCT1-3) were provided by Research Center for Molecular Medicine, Medical University of Vienna, Austria (CEMM) and constructed as reported previously [17 (link)]. HeLa cells were obtained from AddexBio (C0008001, San Diego, CA, USA). Corticosterone, 1-Ethyl-2-[(1-ethyl-2(1H)-quinolinylidene)methyl]quinolinium iodide (decynium-22), 1-Methyl-4-phenylpyridinium iodide (MPP⁺), 4-(4-diethylaminostyryl)-1-methyl-pyridinium iodide (DiASP), doxycycline hyclate, lansoprazole, ouabain, Dulbecco’s modified Eagles medium (DMEM), Minimum Essential Medium Eagle (MEM) and G418 were purchased from Sigma Aldrich (Merck, Darmstadt, Germany). Nilotinib, and ibrutinib were purchased from MedChemExpress (South Brunswick, NJ, USA). Hank’s Balanced Salt Solution (HBSS) and poly-D-lysine were obtained from ThermoFisher Scientific (Waltham, MA, USA). All other chemicals were bought from standard commercial resources and were of analytical grade.