All rats that were used for pharmacological infusions were implanted bilaterally in Prh with 22-gauge indwelling guide cannulas. Subjects were anesthetized with ketamine (Holliday, 74 mg/kg, i.p.) and xylazine (Konig, 7.4 mg/kg, i.p.) and placed in a stereotaxic frame (David Kopf Instruments, Tujunga, CA, USA) with the incisor bar set at −3.2 mm. Guide cannulas were implanted according to the following coordinates, measured relative to the skull at bregma (Paxinos and Watson, 1998 (link)) anteroposterior −5.5 mm, lateral ± 6.6 mm, dorsoventral −7.1 mm. The cannulas were secured to the skull using dental acrylic. Obturators, cut to sit flush with the tip of the guide cannulas and with an outer diameter of 0.36 mm, were inserted into the guides and remained there until the first infusions. At the completion of each surgery, an antibiotic was applied for 3 days (Enrofloxacin; 0.27 mg kg-1, Vetanco, Arg). Animals were given approximately 7 days to recover before behavioral testing and drug infusions.
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