Quantifying Cellular Metabolic States

Mitochondrial NAD(P)H autofluorescence was measured by fluorescence microscopy with an UV filter. Cytosolic NAD⁺/NADH ratio was measured using Peredox^{17 (link)} (Addgene 32383). Total intracellular NAD⁺/NADH ratio was measured using the NAD⁺/NADH Quantitation Kit (BioVision) and the EnzyChrom NAD⁺/NADH Assay Kit (BioAssay Systems). Lactate and pyruvate concentrations were measured using Lactate and Pyruvate Assay Kits (Cayman Chemical).

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Marcu R., Kotha S., Zhi Z., Qin W., Neeley C.K., Wang R.K., Zheng Y, & Hawkins B.J. (2015). The Mitochondrial Permeability Transition Pore Regulates Endothelial Bioenergetics and Angiogenesis. Circulation research, 116(8), 1336-1345.

Publication 2015

NAD+/NADH Quantitation Kit EnzyChrom NAD+/NADH Assay Kit

Assay Cayman Cytosolic Fluorescence microscopy Intracellular Lactate Mitochondrial Nadh Pyruvate

Corresponding Organization :

Other organizations : University of Washington, University of Michigan–Ann Arbor

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