In the study, samples were taken from the control and patient groups after 12 h of fasting into a tube containing aprotinin (BD Vacutainer SST II Advance, BD, Plymouth, UK). Blood samples were centrifuged at 2000–3000 rpm for 20 min and plasma containing Metallothionein-1 was placed in small volume tubes for analysis and stored at −20 °C until the study day.
Plasma Metallothionein-1 levels were studied using the Human Metallothionein-1 ELISA Kit (Bioassay Technology Laboratory, catalog no: E4358hu, Shanghai, China) in accordance with the study procedures specified in the kit catalog; absorbance measurement was made using a Chromate 4300 Microplate Reader (Awareness Technology, Palm City, FL, USA) device. The minimum detection limit of Metallothionein-1 was 0.24 ng/mL. The intra-assay and inter-assay coefficient of variation for plasma Metallothionein-1 were <8% and <10%, respectively.
Plasma Metallothionein-1 levels were studied using the Human Metallothionein-1 ELISA Kit (Bioassay Technology Laboratory, catalog no: E4358hu, Shanghai, China) in accordance with the study procedures specified in the kit catalog; absorbance measurement was made using a Chromate 4300 Microplate Reader (Awareness Technology, Palm City, FL, USA) device. The minimum detection limit of Metallothionein-1 was 0.24 ng/mL. The intra-assay and inter-assay coefficient of variation for plasma Metallothionein-1 were <8% and <10%, respectively.
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