A549 and H1299 cells were seeded in 96-well plates (2 × 103 cells/well). Next, 100 µl of EdU (50 µM/L, KeyGEN, Nanjing, China) was added to the plates for 2 h of incubation. Cells were fixed with 4% paraformaldehyde in phosphate-buffered saline for 30 min. Cell nuclei were subsequently stained with 4ʹ,6-diamidino-2ʹ-phenylindole and then observed with a fluorescence microscope (Leica, Nikon, Olympus, Zeiss) [20 (link)].
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