V. dahliae was inoculated into 4-week-old Arabidopsis thaliana plants by the method of Zhang et al. [24 (link)]. Then the leaves of the transgenic plant were taken at 6, 12, 24, 36, and 48 h, respectively. The activity of H2O2 and SOD was determined according to the kit’s instructions (Jiancheng Biotech Inc., Nanjing, China). The protein content was determined using the BCA protein detection kit (Sangon Biotech, Shanghai, China).
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