Splenic T Cell Isolation from Mice

Splenic T cells were purified from adult (2-6 months) mice as previously described (3 (link), 9 (link)). Briefly, pan-T cells were isolated with a negative selection kit (STEMCELL Technologies, Vancouver, Canada) according to the manufacturer’s protocol. Isolated cells were washed with serum-free RPMI 1640 (Life Technologies, Grand Island, NY) prior to use.

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Arkee T., Hostager B.S., Houtman J.C, & Bishop G.A. (2021). TRAF3 in T cells restrains negative regulators of LAT to promote TCR/CD28 signaling. Journal of immunology (Baltimore, Md. : 1950), 207(1), 322-332.

Publication 2021

negative selection kit RPMI 1640

Adult Cells Mice Serum Splenic Stemcell T cells

Corresponding Organization :

Other organizations : University of Iowa, Iowa City VA Medical Center

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Variable analysis

independent variables

Mice age (2-6 months)

dependent variables

Splenic T cell purity

control variables

Negative selection kit (STEMCELL Technologies)
Serum-free RPMI 1640 (Life Technologies)

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