The anti-VEGFA and anti-FGF2 primary antibodies for ELISA were purchased from Abcam (Cambridge, UK). The ELISA coating solution, TMB Substrate, and ELISA STOP solution were purchased from SolarBio (Beijing, China). The detailed procedures were as described previously 34 (link).
Protocol detail
Quantifying Angiogenic Factors by ELISA
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Anti antibodies
Elisa
Fgf2
Corresponding Organization : Shanghai Jiao Tong University
Other organizations : Shanghai Traditional Chinese Medicine Hospital
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Variable analysis
independent variables
- Anti-VEGFA primary antibody
- Anti-FGF2 primary antibody
- ELISA results
- ELISA coating solution
- TMB Substrate
- ELISA STOP solution
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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