Immunohistochemistry of IGFBP7 and Latexin

Immunohistochemistry using sections of paraffin-embedded tissues was carried out as previously described^{11 (link),19 (link)}. Antibodies used for IGFBP7 and Latexin staining were mouse anti-human IGFBP7 (Abcam) and goat anti-Latexin (R&D) as described for flow cytometry. For IGFBP7 staining, samples were incubated in a steamer for 20 min in S1699 (Dako) prior to incubation with antibody.
Stained slides were scanned by a Mirax microscope (Zeiss, Jena, Germany) and the Panoramic Viewer software (3D Histech, Budapest, Hungary) was used to take images.

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Wicklein D., Otto B., Suling A., Elies E., Lüers G., Lange T., Feldhaus S., Maar H., Schröder-Schwarz J., Brunner G., Wagener C, & Schumacher U. (2018). CEACAM1 promotes melanoma metastasis and is involved in the regulation of the EMT associated gene network in melanoma cells. Scientific Reports, 8, 11893.

Publication 2018

S1699 Mirax microscope Panoramic Viewer software

Antibodies Antibody Embedded paraffin Flow cytometry Goat Human Immunohistochemistry Microscope Mouse Tissues

Corresponding Organization :

Other organizations : University Cancer Center Hamburg, Universität Hamburg, University Medical Center Hamburg-Eppendorf, Eppendorf (Germany), Fachklinik Hornheide

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Variable analysis

independent variables

Antibodies used for IGFBP7 and Latexin staining: mouse anti-human IGFBP7 (Abcam) and goat anti-Latexin (R&D)

dependent variables

IGFBP7 and Latexin staining

control variables

Immunohistochemistry using sections of paraffin-embedded tissues was carried out as previously described^{11 (link),19 (link)}

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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