Apoptosis Assay with Annexin V and PI

Apoptosis was assessed by the Tali™ apoptosis assay kit (Invitrogen™, Life Technologies, Monza, Italy), which uses Annexin V Alexa Fluor™ 488 (Invitrogen™, Life Technologies, Monza, Italy) and propidium iodide (Invitrogen™, Life Technologies, Monza, Italy) to differentiate cells as live, dead, or apoptotic as previously indicated [24 (link)]. Each treatment was performed in three replicates and the final results were reported as a fold increase compared to the control.

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Giampieri F., Gasparrini M., Forbes-Hernández T.Y., Manna P.P., Zhang J., Reboredo-Rodríguez P., Cianciosi D., Quiles J.L., Torres Fernández-Piñar C., Orantes-Bermejo F.J., Bompadre S., Afrin S, & Battino M. (2018). Beeswax by-Products Efficiently Counteract the Oxidative Damage Induced by an Oxidant Agent in Human Dermal Fibroblasts. International Journal of Molecular Sciences, 19(9), 2842.

Publication 2018

Tali™ apoptosis assay kit Annexin V Alexa Fluor™ 488 propidium iodide

Alexa fluor 488 Annexin v Apoptotic Assay Cells Propidium iodide Tali

Corresponding Organization : Marche Polytechnic University

Other organizations : Universidade de Vigo, Universidad de Granada

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Variable analysis

independent variables

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Apoptosis

control variables

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Dead cells
Apoptotic cells

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