Cells in 6-well plates (1.0 × 106 cells/well) were incubated for 24 h then treated with JAS239 or MN58b. After 1 h, cells were spiked for 1 h with 0.5 μCi/mL [methyl-14C]-choline (PerkinElmer) and fixed with trichloroacetic acid. Aqueous cellular extracts were separated by thin layer chromatography (TLC) and the 14C-PC production measured by autoradiography on a Fujifilm FLA-7000 using previously described methods [4 (link), 23 (link)].
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