The paraffin retinal sections were processed according to IHC protocol and previous study (17 (link)). Photos were taken under an optical microscope (OLYMPUS, CX-21, Japan). Primary antibodies were as follows: JAK2 (bs-23003r, Bioss), STAT3 (60199-1-lg, Proteintech), p-JAK2 (ab32101, Abcam), p-STAT3 (sc-8059, Santa), Bcl-2 (ab194583, Abcam), and Bax (A19684, ABclonal). HRP-conjugated secondary antibodies were AS-1107 and AS-11069 (Aspen).
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