All animal experiments were carried out in accordance with the guidelines of the German Animal Welfare Act and approved by the local authorities (Landesamt für Natur, Umwelt und Verbraucherschutz Nordrhein-Westfalen). Animals were socially housed under a fixed 12:12 h light/darkness cycle with ad libitum access to food and water.
A total of 15 adult, male NMRI-Foxnlnu/nu mice (12–14 weeks old, 32–37 g; Janvier, France) were used in this study. All surgical experiments were performed under anesthesia with 1.5–2% Isoflurane in a mixture of 70/30% N2O/O2 atmosphere. Analgetic treatment included subcutaneous injection of 4 mg/kg Carprofen (Rimadyl, Zoetis, Berlin, Germany) twice a day, for 3 days following surgery. Two days after stroke induction by the filament occlusion method (18 (link), 23 (link)), the animals received an intracortical stem cell implantation on the ipsilateral hemisphere. Resting state fMRI was performed 1 week before, as well as 1, 2, 4, 8, and 12 weeks after stroke induction and cell implantation (2 days after stroke induction), while vitality of the transgenic graft was monitored by BLI at weeks 2, 3, 4, 5, 7, 9, 10, and 12 after stroke.
A total of 15 adult, male NMRI-Foxnlnu/nu mice (12–14 weeks old, 32–37 g; Janvier, France) were used in this study. All surgical experiments were performed under anesthesia with 1.5–2% Isoflurane in a mixture of 70/30% N2O/O2 atmosphere. Analgetic treatment included subcutaneous injection of 4 mg/kg Carprofen (Rimadyl, Zoetis, Berlin, Germany) twice a day, for 3 days following surgery. Two days after stroke induction by the filament occlusion method (18 (link), 23 (link)), the animals received an intracortical stem cell implantation on the ipsilateral hemisphere. Resting state fMRI was performed 1 week before, as well as 1, 2, 4, 8, and 12 weeks after stroke induction and cell implantation (2 days after stroke induction), while vitality of the transgenic graft was monitored by BLI at weeks 2, 3, 4, 5, 7, 9, 10, and 12 after stroke.
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