Albuminuria was assessed by ELISA according to the manufacturer’s instructions (Bethyl Laboratories, Inc., Montgomery, TX, United States) (Watson et al., 2012 (link)).
Cystatin C (BioVendor – Laboratorni medicina a.s., Karasek, Brno, Czech Republic) and KIM-1 (kidney injury molecule-1, or hepatitis A virus cellular receptor 1; Assay Matrix; EIAab, Optics Valley, Wuhan, China) ELISA analyses were carried out on plasma samples as per the manufacturer’s instructions.
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