Retinal Imaging in Rodents

Mice were imaged using a Spectralis OCT as previously described (45 (link)). Rats were imaged using the Bioptigen Spectral-domain ophthalmic imaging system, as previously described (39 (link)). Briefly, image acquisition was obtained by using the rectangular scanning protocol consisting of a 2 mm × 2 mm perimeter with 750 A-scans (lines) and 5 B-scans (frames) with 60 frames/B-scan (for rat retina) or 1.4 mm × 1.4 mm perimeter with 750 A-scans (lines) and 10 B-scans (frames) with 20 frames/B-scan (for mouse retina). The Bioptigen InVivoVue Diver 2.0 was used to enable manual segmentation of the retinal layers and the ONL thickness was measured after exporting results from Diver to Excel. Statistical analysis was performed using two-tailed Student’s t-test for samples with unequal variance, except for the spider plots, which were analyzed by two-way analysis of variance with Sidak correction.