Sixty-two conventional clinical and targeted metabolites were quantitatively assayed, as previously described [15 (link)]. Briefly, levels of conventional clinical metabolites (lactate, triglycerides, 3-hydroxybutyrate, glycerol, and NEFA) were measured using a Unicel DxC 600 clinical analyzer (Beckman Coulter, Brea, CA, USA). Targeted metabolomic assays for acylcarnitines and amino acids were performed via tandem mass spectrometry (MS), for which known quantities of stable isotope-labelled internal standards were added using an Acquity TQD Triple Quadrupole system (Waters Corporation, Milford, MA, USA).
Free full text: Click here
