Liposomal Doxorubicin Formulation and Characterization

We prepared liposomes using the thin-lipid-film-hydration method following previous publications [17 (link),18 (link)]. In brief, the lipids were dissolved in dichloromethane (Daejung, Siheung-si, Gyeonggi-do, Korea), and the solvent was removed. Then, the film of lipids was dispersed in the distilled water containing doxorubicin hydrochloride (LC laboratories, Woburn, MA, USA) by sonication. Then, removal of unloaded doxorubicin was done through dialysis for one hour. We analyzed the amount of doxorubicin in the liposomes by measuring the fluorescence of doxorubicin (490/570 nm) after liposomes were completely disassembled by Triton X-100 (Samchun, Pyeongtaek-si, Gyeonggi-do, Korea).

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Lee H., Son J., Min S.K., Na C.B., Yi G., Koo H, & Park J.B. (2019). A Study of the Effects of Doxorubicin-Containing Liposomes on Osteogenesis of 3D Stem Cell Spheroids Derived from Gingiva. Materials, 12(17), 2693.

Publication 2019

doxorubicin hydrochloride doxorubicin Triton X-100

Dialysis Dichloromethane Doxorubicin Doxorubicin hydrochloride Fluorescence Lipids Liposomes Solvent Triton x 100

Corresponding Organization : Catholic University of Korea

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Variable analysis

independent variables

Thin-lipid-film-hydration method for liposome preparation

dependent variables

Amount of doxorubicin loaded into the liposomes
Fluorescence of doxorubicin (490/570 nm) after liposomes were disassembled by Triton X-100

control variables

Lipids dissolved in dichloromethane
Removal of dichloromethane to form a lipid film
Dispersion of lipid film in distilled water containing doxorubicin hydrochloride
Sonication of the mixture
Removal of unloaded doxorubicin through dialysis for one hour

controls

Positive control: Not mentioned
Negative control: Not mentioned

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