Quantification of CSF NFL was performed with
the Uman Diagnostics NF-Light ELISA kit (Umea, Sweden) according to
the manufacturer’s protocol using a final dilution factor of
1:2. CSF and serum YKL-40 levels were also quantified according to
the manufacturer’s protocol for YKL-40 (Invitrogen, Thermo
Fisher Scientific) with dilution factors of 1:200 and 1:150 for CSF
and serum, respectively. All samples were subjected to the same freeze–thaw
cycle to ensure comparability of the results. In addition, samples
were randomized to nullify potential differences due to a batch effect
in all analyses and an edge effect during ELISA.
the Uman Diagnostics NF-Light ELISA kit (Umea, Sweden) according to
the manufacturer’s protocol using a final dilution factor of
1:2. CSF and serum YKL-40 levels were also quantified according to
the manufacturer’s protocol for YKL-40 (Invitrogen, Thermo
Fisher Scientific) with dilution factors of 1:200 and 1:150 for CSF
and serum, respectively. All samples were subjected to the same freeze–thaw
cycle to ensure comparability of the results. In addition, samples
were randomized to nullify potential differences due to a batch effect
in all analyses and an edge effect during ELISA.
