Antifungal Activity of Essential Oils

The agar disc diffusion method described by Adukwu et al. (2016 (link)) was used for determination of the antifungal properties of CEOs. Miconazole (Sigma, USA) with the concentration at 10 μg/mL dissolved in DMSO (Sigma) was used as the reference antifungal drug. Briefly, 100 μL of yeast suspension was spread onto SDA plates and then paper discs (6 mm, Sigma) impregnated with 10 μL of essential oils or dissolved Miconazole were placed over the agar surface, and plates were incubated at 30 °C for 48 h. Plates with discs in the absence of any treatment were used as reference controls. The diameter of the resulting inhibition zone in the fungal lawn was measured in millimetres.