Western blotting was performed as previously described [32 (link), 33 (link)]. The following antibodies were used: anti-TNFSF11 (RANKL) (Abcam, ab9957); anti-Catalase (Cell Signaling Technology, 12,980); anti-GPX4 (Cell Signaling Technology, 52,455), anti-SOD1 (Proteintech, 67480-1-Ig); anti-4 Hydroxynonenal (Abcam, ab48506), anti-caspase 3 (Cell Signaling Technology, 9962), anti-caspase 9 (Cell Signaling Technology, 9502) and anti-α-Tubulin (Proteintech, 66031-1-Ig). Image J software was used for the quantitative analysis of each band, the relative protein expression was normalized with α-Tubulin.
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