Taxol/paclitaxel were purchased from Sigma-Aldrich, Inc., and the dry powder was prepared for 100 μM stock solutions in DMSO. Tissue culture flasks (trade mark Falcon), tissue culture media, trypsin, and 100X antibiotic-antimycotic solution (Cellgro, Mediatech, Inc) were purchased from VWR Inc. (Springfield, NJ). Alexa Fluor 488 and 555 conjugated secondary antibodies were purchased from Life Sciences Inc. (Eugene, Oregon) for use in immunofluorescence microscopy. Primary antibodies: anti-Lamin A (1:400, H-102, rabbit polyclonal IgG); mouse monoclonal anti-Lamin B (1:300, sc373918); and goat polyclonal anti-Lamin B (1:400, sc6216) were from Santa Cruz Biotechnology Inc.; rabbit polyclonal anti-Lamin B1 (1:1000, ab16048) and mouse monoclonal anti- αTubulin (1:500, 66,031) were from Abcam and Proteintech, respectively. Mouse monoclonal anti-alpha-tubulin, were purchased from Sigma-Aldrich, Inc. (St. Louis, MO). More details of the reagents used are also reported previously [59 (link)–62 (link)].
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