Fetal Brain Tissue Acquisition and Processing

Tissue was provided by the Birth Defects Research Laboratory (BDRL) at the University of Washington and Advanced Bioscience Resources Incorporated (ABR; Alameda, CA). All work was performed according to guidelines for the research use of human brain tissue (ABR) or the UAGA and NOTA guidelines for the acquisition and distribution of human tissue for bio-medical research purposes (BDRL) and with approval by the Human Investigation Committees and Institutional Ethics Committees of each institute from which samples were obtained. Appropriate written informed consent was obtained and all available non-identifying information was recorded for each sample. Specimens for microarray profiling consisted of two 21 pcw females, one 15 pcw male and one 16 pcw female (Suppl. Table 1).

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Miller J.A., Ding S.L., Sunkin S.M., Smith K.A., Ng L., Szafer A., Ebbert A., Riley Z.L., Aiona K., Arnold J.M., Bennet C., Bertagnolli D., Brouner K., Butler S., Caldejon S., Carey A., Cuhaciyan C., Dalley R.A., Dee N., Dolbeare T.A., Facer B.A., Feng D., Fliss T.P., Gee G., Goldy J., Gourley L., Gregor B.W., Gu G., Howard R.E., Jochim J.M., Kuan C.L., Lau C., Lee C.K., Lee F., Lemon T.A., Lesnar P., McMurray B., Mastan N., Mosqueda N.F., Naluai-Cecchini T., Ngo N.K., Nyhus J., Oldre A., Olson E., Parente J., Parker P.D., Parry S.E., Player A.S., Pletikos M., Reding M., Royall J.J., Roll K., Sandman D., Sarreal M., Shapouri S., Shapovalova N.V., Shen E.H., Sjoquist N., Slaughterbeck C.R., Smith M., Sodt A.J., Williams D., Zöllei L., Fischl B., Gerstein M.B., Geschwind D.H., Glass I.A., Hawrylycz M.J., Hevner R.F., Huang H., Jones A.R., Knowles J.A., Levitt P., Phillips J.W., Sestan N., Wohnoutka P., Dang C., Bernard A., Hohmann J.G, & Lein E.S. (2014). Transcriptional Landscape of the Prenatal Human Brain. Nature, 508(7495), 199-206.

Publication 2014

Birth defects Brain Female Human Institutional ethics committees Male Microarray Nota Tissue

Corresponding Organization : Allen Institute

Other organizations : University of Washington, Athinoula A. Martinos Center for Biomedical Imaging, Harvard University, Massachusetts General Hospital, Yale University, Seattle University, Neurological Surgery, The University of Texas Southwestern Medical Center, University of Southern California, Children's Hospital of Los Angeles

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Gene expression profiles measured using microarray analysis

control variables

Gestational age (21 pcw females, 15 pcw male, 16 pcw female)
Sex (2 females, 1 male)

controls

Positive control: None mentioned
Negative control: None mentioned

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