Spatholobi Caulis Bioactivities Analysis

Anti-phospho-acetyl-CoA carboxylase (ACC), anti-phospho-AMPKα, anti-phospho-LKB1, anti-Phospho-LATS1, anti-phospho-YAP, anti-YAP, anti-Poly (ADP-ribose) polymerases (PARP), anti-caspase-3, anti-B-cell lymphoma-extra-large (Bcl-xL), and anti-β-actin were purchased from Cell Signaling Technology (Danvers, MA, USA). HRP-conjugated anti-rabbit IgG and HRP-conjugated anti-mouse IgG were bought from Enzo Life Sciences (Farmingdale, NY, USA). Polyethylene glycol (PEG) 400 is manufactured by Yakury Pure Chemical Co., Ltd. (Kyoto, Japan). SC was produced by water extraction of the medicinal herb, Spatholobi Caulis (Daewon pharmacy, Daewon, Korea) as previously described [17 (link),19 (link)]; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), rhodamine 123 (Rh123), 2′,7′-dichlorofluorescein diacetate (DCFH-DA), arachidonic acid (AA), ferric nitrilotriacetic acid (iron), Harris hematoxylin and eosin (H&E), and other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).