Glioblastoma and Oligodendroglioma Cell Culture

The patient-derived glioblastoma cell lines S24, T269, T325 and P3xx and BT088 oligodendroglioma cells were kept under stem-like neurosphere conditions (37 °C, 5.0% CO₂): DMEM F-12 medium (31330-038, Invitrogen), B27 supplement (12587-010, Invitrogen), 5 μg ml⁻¹ insulin (I9278, Sigma-Aldrich), 5 μg ml⁻¹ heparin (H4784, Sigma-Aldrich), 20 ng ml⁻¹ epidermal growth factor (rhEGF; 236-EG, R&D Systems), and 20 ng ml⁻¹ basic fibroblast growth factor (bFGF; PHG0021, Thermo Fisher Scientific). S24 (human, female), T269 (human, male), T325 (human, male), and P3xx (human) were authentificated (Multiplexion GmbH, Germany). S24, T269, and T325 were further authenticated as glioblastoma by Illumina 850k methylation array^{56 (link)}. BT088 cells were obtained from ATCC (ATCC CRL-3417, RRID:CVCL_N708) (human, male). The molecular characterization of all used cancer cell lines can be found in Supplementary Tables S1 and S2.