Seven tissue samples from of SCC and seven samples of healthy cervical tissue were used for microarray testing. Additionally, cervical tissue samples from 11 cases of chronic cervicitis, 16 cases of low-grade squamous intraepithelial lesion (LSIL), 20 cases of high-grade squamous intraepithelial lesion (HSIL), and 21 cases of cervical cancer were used to detect hsa_circ_0000276 expression. All samples were single HPV16-positive. From January 2019 to January 2022, samples were obtained from patients who underwent colposcopy at the Second Hospital of the Shanxi Medical University. The tissue specimens were independently diagnosed by two experienced clinical pathologists.
The inclusion criteria were as follows: (i) married women aged ≤ 65 years; (ii) residents of Taiyuan for > 1 year; and (iii) written informed consent provided. The exclusion criteria were as follows: (i) pregnant women; (ii) a history of hysterectomy; (iii) a history of treatment of cervical and vaginal diseases; and (iv) presence of other malignant tumors. Written informed consent was obtained from all study participants or their legal guardians. The Ethics Review Committee of the Second Hospital of the Shanxi Medical University approved this study [approval number (2019) YX No. (280)].
The operational protocol for cervical tissue collection was as follows. The doctor who performed the examination had > 2 years of experience. The lesion site was assessed, and if cervical cancer was considered, tissue was collected from: (1) the cancer site (two pieces of tissue [each approximately 5 mm in size] were clamped) and (2) the matching normal tissues (two pieces of tissues [each approximately 5 mm in size] were clamped at approximately 3–5 cm from the cancer site). After clamping, the tissues were placed in 10% neutral formalin for 6–12 h for fixation and processed within 24 h for routine pathological examination. The tissues were then collected in pre-cooled tubes and quickly snap frozen in liquid nitrogen.
The inclusion criteria were as follows: (i) married women aged ≤ 65 years; (ii) residents of Taiyuan for > 1 year; and (iii) written informed consent provided. The exclusion criteria were as follows: (i) pregnant women; (ii) a history of hysterectomy; (iii) a history of treatment of cervical and vaginal diseases; and (iv) presence of other malignant tumors. Written informed consent was obtained from all study participants or their legal guardians. The Ethics Review Committee of the Second Hospital of the Shanxi Medical University approved this study [approval number (2019) YX No. (280)].
The operational protocol for cervical tissue collection was as follows. The doctor who performed the examination had > 2 years of experience. The lesion site was assessed, and if cervical cancer was considered, tissue was collected from: (1) the cancer site (two pieces of tissue [each approximately 5 mm in size] were clamped) and (2) the matching normal tissues (two pieces of tissues [each approximately 5 mm in size] were clamped at approximately 3–5 cm from the cancer site). After clamping, the tissues were placed in 10% neutral formalin for 6–12 h for fixation and processed within 24 h for routine pathological examination. The tissues were then collected in pre-cooled tubes and quickly snap frozen in liquid nitrogen.
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