Synergistic Anti-Cancer Effects of AF and ICG-001

AF, ICG-001, and N-acetyl cysteine (NAC) were purchased from MedChemExpress Inc. (Monmouth Junction, NJ). For co-treatment, the cells were incubated with AF and ICG-001 premixed under the dose ratio of 1:10 (2 μM AF, 20 μM ICG-001, 2 μM AF + 20 μM ICG-001) for 24 h; for the experiments regarding the N-acetyl cysteine (NAC) inhibition, the cells were pre-treated with 5 mM NAC dissolved in culture medium for 1 h before drug treatment (17 (link)); for the mice co-treatment, mice were treated with AF and ICG-001 premixed under the dose ratio of 1:2 (5 mg/kg AF, 10 mg/kg ICG-001, 5 mg/kg AF + 10 mg/kg ICG-001) for 21 days (39 (link)).

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Lin Z., Lin Z., Zhao Y., Cheng N., Zhang D., Lin J., Zhang H, & Lin D. (2021). Auranofin and ICG-001 Emerge Synergistic Anti-tumor Effect on Canine Breast Cancer by Inducing Apoptosis via Mitochondrial Pathway. Frontiers in Veterinary Science, 8, 772687.

Publication 2021

ICG-001 N-acetyl cysteine (NAC)

Acetyl cysteine n Cells Culture medium Drug Icg 001 Inhibition Mice

Corresponding Organization :

Other organizations : China Agricultural University, Hainan University

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Variable analysis

independent variables

AF and ICG-001 co-treatment with a dose ratio of 1:10 (2 μM AF, 20 μM ICG-001, 2 μM AF + 20 μM ICG-001) for 24 h
N-acetyl cysteine (NAC) pre-treatment at 5 mM for 1 h before drug treatment
AF and ICG-001 co-treatment with a dose ratio of 1:2 (5 mg/kg AF, 10 mg/kg ICG-001, 5 mg/kg AF + 10 mg/kg ICG-001) for 21 days in mice

dependent variables

Not explicitly mentioned

control variables

Cell culture medium
Not explicitly mentioned for the in vivo experiments

controls

Positive control: Not mentioned
Negative control: Not mentioned

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